NMN & TMG Bundle: what the latest science (2026) says about this longevity combination

Anyone delving into longevity supplements quickly encounters two names that are increasingly mentioned together: NMN and TMG. Separately, they are both well-known names in the world of healthy aging, but in recent years the combination has appeared more frequently — including in the NMN & TMG Bundle of Every Day Better. In this article, we dive into the science: what do NMN and TMG do individually, why are they often combined, and what do the most recent studies from 2025 and 2026 actually say about it? We keep it factual and sober: supplement research never stands still, and it is important to distinguish between promising signals and proven effects.

What exactly is NMN?

NMN, or nicotinamide mononucleotide, is a molecule that occurs naturally in the body and serves as a building block for NAD⁺ (nicotinamide adenine dinucleotide). NAD⁺ is a coenzyme present in virtually every cell and plays a key role in energy production, DNA repair, and cellular metabolism. As we age, NAD⁺ levels in the body steadily decline — a fact that has been known for some time from animal studies and forms the basis of much of the current NMN research.

The idea behind NMN supplements is simple: by taking NMN orally, the body would receive more building blocks to produce NAD⁺, with the goal of (partially) supporting the naturally declining NAD⁺ levels. On the NMN knowledge page and the page about how NMN works, we go deeper into the underlying mechanism; here we focus primarily on what the most recent research shows.

The latest scientific insights on NMN in 2026

The NMN research field has matured significantly in recent years. While the early years relied mainly on mouse studies, several controlled human studies have now been published — and in 2026, new review studies have been added that summarize this data.

A critical look at the state of affairs

A review published in 2026 in the Journal of Food Composition and Analysis, “Unraveling nicotinamide mononucleotide (NMN): A critical review of health implications, synthesis pathways, and analytical techniques”, maps out the current state of affairs regarding NMN. The conclusion is nuanced: NMN shows a favorable safety profile and moderate effectiveness on various health markers in animal studies and early human studies, but the authors emphasize that the optimal dosage, bioavailability, and translation to clinical practice have not yet been sufficiently crystallized. In short: promising, but not yet a settled matter.

That same nuance is reflected in a large systematic review published in 2026 in Ageing Research Reviews, titled “NAD⁺ supplementation for anti-aging and wellness: A PRISMA-guided systematic review of preclinical and clinical evidence” (also available via ScienceDirect). This PRISMA-structured review collects and assesses both preclinical and clinical evidence for NAD⁺-boosting supplements such as NMN and NR. The authors conclude that there is now consistent evidence that oral NMN intake increases NAD⁺ levels in the blood and that the supplements are generally well-tolerated, but that convincing evidence for hard outcomes such as increased lifespan in humans is still lacking. For 2026, that is a fair summary of where the field stands: the biochemistry works, but the translation to tangible health gains is still very much a subject of research.

What do controlled human studies show?

In addition to these review studies, directly controlled human studies have also been published in recent years that provide a good picture of what NMN does and does not (yet) demonstrably do.

A double-blind, randomized, placebo-controlled study published in GeroScience, “Ingestion of β-nicotinamide mononucleotide increased blood NAD levels, maintained walking speed, and improved sleep quality in older adults”, followed older participants for twelve weeks. The results: a clear increase in NAD-related metabolites in the blood, maintained walking speed in the NMN group (while it decreased in the placebo group), and improved sleep quality in the participants who received NMN. This is one of the first studies to show that an increased NAD⁺ status may be related to functional outcomes in the elderly, rather than just blood values.

Furthermore, two broader meta-analyses confirm the image that NMN is generally safe for use in adults. The systematic review and meta-analysis “Safety and Metabolism-Related Outcomes of Oral Nicotinamide Mononucleotide Supplementation in Adults”, published in Nutrients, bundles safety data from multiple controlled studies and concludes that oral NMN supplementation at the studied dosages shows no serious side effects. A second meta-analysis, “Efficacy of oral nicotinamide mononucleotide supplementation on glucose and lipid metabolism for adults”, looked specifically at effects on glucose and lipid metabolism and found indications of favorable shifts in some of the metabolic markers studied, although the authors also emphasize here that more large-scale research is needed to confirm this.

Finally, a double-blind, placebo-controlled study appeared on medRxiv in 2025, “A Novel NAD+ Supporting Supplement Increased NAD+ Levels, Improved Well-Being, and Alleviated Aging Symptoms”, which showed that NAD⁺-supporting supplementation not only affects blood values but was also associated in self-reports with improved overall well-being and fewer perceived signs of aging. As a preprint, this study has not yet been fully peer-reviewed, but it aligns with the broader trend described in the previously mentioned reviews. Those who want to follow the full line of research from the past year will find a more extensive overview on our page about the most important NMN studies.

For those wondering how best to dose NMN based on these studies, we have a separate page on NMN dosage, and for possible side effects, we refer to our page on NMN side effects.

What is TMG and why does it belong in an NMN stack?

TMG, or trimethylglycine (also known as betaine), is a substance that occurs naturally in foods such as beets, spinach, and whole-grain products. TMG is a so-called methyl donor: it provides methyl groups that the body uses in numerous biochemical processes, including the conversion of homocysteine to methionine. This latter process — methylation — is important for liver metabolism, cell division, and the production of various endogenous substances, among other things. More background on this can be found on our TMG knowledge page.

The reason TMG is relevant in combination with NMN lies in the biochemistry. When the body converts NMN into NAD⁺ and subsequently breaks it down, nicotinamide is formed, among other things, which is converted and removed via a process called methylation. This methylation process consumes methyl groups — the same methyl groups the body also needs for other essential processes, including homocysteine metabolism. The underlying idea behind combining NMN with a methyl donor such as TMG is that an extra supply of methyl groups can help offset this consumption. This is a mechanistic hypothesis that is frequently cited in the supplement world; however, direct comparative research testing NMN with and without TMG in humans is still scarce, and it is important to state that honestly.

The latest science on TMG and betaine

Even apart from the combination with NMN, TMG is a substance that has been the subject of interesting research in recent years, particularly in the areas of liver metabolism and homocysteine.

A 2025 pilot study, “Pilot trials of oral betaine in participants with metabolic dysfunction-associated steatotic liver disease and elevated alanine aminotransferase”, investigated oral betaine supplementation in participants with fatty liver (MASLD) and elevated ALT levels — a marker for liver stress. These pilot studies build on previous research into betaine in liver diseases and provide additional indications that betaine supplementation may be relevant for supporting liver metabolism, although this is explicitly pilot research in a specific patient group and not a statement for the general population.

For the effect of betaine on metabolic markers in a broader group, the randomized clinical trial “Metabolic Effects of Betaine: A Randomized Clinical Trial of Betaine Supplementation in Prediabetes”, published in The Journal of Clinical Endocrinology & Metabolism, is relevant. This study investigated betaine supplementation in people with prediabetes and looked at a range of metabolic outcomes. The results were mixed: betaine lowered homocysteine levels as expected based on the mechanism of action, but the researchers also found some less favorable shifts in certain lipid values, illustrating that the effect of TMG on metabolism is context-dependent and not simply positive across the board.

This nuanced view is also confirmed in the narrative review “Decoding Betaine: A Critical Analysis of Therapeutic Potential Compared with Marketing Hype”. The authors critically contrast the scientific basis of betaine with the way it is often presented in the supplement market, concluding that while there is solid mechanistic evidence for TMG’s role in methylation and homocysteine management, some commercial claims go beyond what current evidence justifies. That is exactly why we prefer to be too cautious rather than too assertive on this page about what TMG does and does not do. More details on the line of research into TMG can be found on our page TMG research, and practical information on dosage on the page TMG dosage.

Why combine NMN and TMG in one bundle?

The logic behind the NMN & TMG Bundle follows directly from the biochemistry described above. NMN is converted into NAD⁺ in the body, and that process generates methylation products that require methyl groups. TMG is one of the most direct and well-researched ways to replenish methyl groups. By taking both supplements together, the bundle aligns with what is often called the “NMN stack” in the longevity community — an approach that is also reflected on our page about the NMN-TMG-GlyNAC longevity stack, where we discuss the combination of multiple cell-targeted supplements.

It is important to emphasize that the vast majority of the research summarized above has studied NMN and TMG separately. Direct clinical research into the combination of both substances together — specifically an NMN+TMG stack tested against NMN alone — is still scarce in the scientific literature of 2025 and 2026. The reasoning for combining them is firmly grounded in known biochemical pathways, but that is different from a proven, measured effect of the combination itself. Anyone looking for the most current, scientifically substantiated approach to healthy aging is therefore well-advised to continue following the individual lines of research for both substances — something we continuously pay attention to on the page about healthy aging.

Contents, dosage, and use of the bundle

The NMN & TMG Bundle contains 60 NMN capsules of 250 mg with a purity of more than 99.5%, and 60 TMG capsules of 500 mg in vegetable HPMC capsules. Both products are suitable for a vegan diet. With a typical dosage of 1 to 2 capsules per day of each product, the bundle lasts approximately 30 to 60 days, depending on the chosen dosage.

The advice is to take 1 to 2 NMN capsules and 1 to 2 TMG capsules daily, preferably during or after a meal. This aligns with the way many of the studies discussed above administered NMN and betaine to participants. For those who prefer to start separately, the NMN capsules and TMG capsules are also available individually; the bundle is primarily intended for those who want to use the combination from the start without having to place two separate orders.

Quality and purity

Given the relatively young status of the NMN research field, the quality of the raw material used is at least as important as the dosage. The NMN in this bundle is independently tested for purity; the corresponding certificates and test results can be found on our page with NMN certificates. This type of independent verification is relevant because the NMN market has grown rapidly in recent years, and not every product on the market offers the same level of transparency regarding purity and composition.

Safety: what does the research say?

Based on current literature, including the aforementioned meta-analysis in Nutrients, oral NMN supplementation at the studied dosages generally appears to be well-tolerated by healthy adults, with no serious short-term side effects reported. A similar picture applies to TMG: it is a substance that occurs naturally in food and is considered relatively safe in research, although some participants in studies report mild digestive complaints at higher dosages.

That being said: neither substance has been extensively studied in pregnant or breastfeeding women, children, or people with specific medical conditions, and long-term data (multiple years) in humans is still largely lacking. If you are using medication, are pregnant, breastfeeding, or have an underlying condition, consult a doctor first before starting NMN or TMG supplements. More specific information about possible side effects is on our pages about NMN side effects and TMG side effects.

Who is the NMN & TMG Bundle interesting for?

This bundle is particularly interesting for people who structurally delve into healthy aging and who want to align with the underlying biochemistry of NAD⁺ supplementation, including the methylation side of the story. It is not a quick fix and not a replacement for a varied diet, sufficient exercise, and good sleep — factors that prove to be at least as important in virtually every study on aging. Those who want to read more broadly about which supplements are considered relevant within the longevity world in 2026 will find an overview on our page longevity supplements in 2026.

Conclusion

The science surrounding NMN and TMG is still moving forward in 2026. Recent reviews in Ageing Research Reviews and the Journal of Food Composition and Analysis, along with controlled studies in GeroScience and meta-analyses in Nutrients, paint a consistent picture: NMN demonstrably increases NAD⁺ status in the body and is generally well-tolerated, with early indications of functional benefits such as maintenance of walking speed and improved sleep quality in the elderly — although hard evidence for effects on lifespan in humans remains absent. For TMG, recent studies in JCEM and pilot research into liver metabolism show that the substance plays a real role in methylation and homocysteine management, with the caveat that not every effect is unambiguously positive.

The combination of both in the NMN & TMG Bundle is based on a logical, biochemically substantiated reasoning, even though direct research into the combination itself is still limited. For those interested in this, the advice remains: start with a realistic dosage, pay attention to quality and purity, and keep an eye on scientific developments — we do that for you on this site, including via our NMN knowledge page and TMG knowledge page. View the full range in our webshop, or contact us if you have questions.

This article is intended for information purposes only and does not constitute medical advice. Supplements are not a substitute for a varied and balanced diet and a healthy lifestyle. In case of doubt, medication use, pregnancy, or underlying conditions, always consult a doctor before starting new supplements.